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2D HET2DJ Experiment

This two-dimensional absolute-value HET2DJ spectrum shows (decoupled) ¹³C chemical shifts on one 2D axis and ¹³C-¹H coupling constants on the other 2D axis. Before setting up the HET2DJ experiment, see Acquiring and Processing 2D Experiments. See the Varian Guide to NMR Experiments for a complete description of this experiment.

Acquiring the experiment:

1. Join the experiment in which the ¹³C reference spectrum was collected. Type het2dj.

2. Type dg. Adjust the parameters listed below for the standard HET2DJ:

sfrq=100.559 dn=H1

tn=C13 dof = tof from ¹H reference spectrum

at = set by spectrometer dm='nny'

np=2048 dmm='wcw'

sw = sw from ¹³C reference spectrum dmf=9900

ss=2 dpwr=40

tpwr = listed at console sw1 = sw from ¹H reference spectrum

pw = ¹³C 90° pulse width ni = multiple of 64

pp = ¹H decoupler 90° pulse width dp='n'

pplvl=49 hs='n'

d1 = ¹H relaxation delay (1-2 *¹H T₁) or ~1.5 rf1 = rf from ¹H reference spectrum

d2=0 rfp1 = rfp from ¹H reference spectrum

tof = tof from ¹³C reference spectrum wp1 = wp from ¹H reference spectrum

nt = multiple of 16 sp1 = sp from ¹H reference spectrum

ct = set by computer temp = 'y' su

make sure spinner is off au to start experiment

Change ni, nt, and d1 to suit your scheduled time. The vertical (non-acquisition) resolution equals sw1/ni. Typical values are listed below for sw1=25000:

d1
ni
sw1
nt
time
vert. res
d1
ni
sw1
nt
time
vert. res

1.5
32
128
16
0:14
4.0 Hz
1.5
32
128
64
0:57
4.0 Hz

1.5
96
128
16
0:49
1.33
1.5
96
128
64
3:17
1.33

1.5
192
128
16
1:58
0.67
1.5
192
128
64
7:51
0.67

1.5
32
512
16
0:13
16.0
1.5
32
512
64
0:54
16.0

1.5
96
512
16
0:42
5.33
1.5
96
512
64
2:49
5.33

1.5
192
512
16
1:29
1.50
1.5
192
512
64
5:56
1.50

Processing, Displaying, and Plotting:

Process using the procedure for Processing a 2D Absolute-Value Experiment. Plot Pos. only contours.

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Last updated: April 1st, 1998
URL: http://nmr.chem.indiana.edu/NMRguide/2dexpt/het2dj.html
Copyright 1996, The Trustees of Indiana University

d1	ni	sw1	nt	time	vert. res	d1	ni	sw1	nt	time	vert. res
1.5	32	128	16	0:14	4.0 Hz	1.5	32	128	64	0:57	4.0 Hz
1.5	96	128	16	0:49	1.33	1.5	96	128	64	3:17	1.33
1.5	192	128	16	1:58	0.67	1.5	192	128	64	7:51	0.67
1.5	32	512	16	0:13	16.0	1.5	32	512	64	0:54	16.0
1.5	96	512	16	0:42	5.33	1.5	96	512	64	2:49	5.33
1.5	192	512	16	1:29	1.50	1.5	192	512	64	5:56	1.50