This two-dimensional phase-sensitive INADEQUATE experiment correlates directly-bonded carbons. Before setting up the INADEQUATE experiment, see Acquiring and Processing 2D Experiments. See the Varian Guide to NMR Experiments for a complete description of this experiment.

Sample Requirements: To obtain reasonable results within 24 hours, a 1-pulse ¹³C spectrum with nt=1 should present a signal/noise ratio of at least 25:1 (position the cursors in an empty region of the 1D spectrum and type dsn).

Acquiring the experiment:

1. Join the experiment in which the ¹³C reference spectrum was collected. Type inadqt.

2. Type dg. Adjust the parameters listed below for the standard INADEQUATE:

sfrq=100.559 dn=H1 dm='n'

tn=C13 dof = tof from ¹H reference spectrum

at = set by spectrometer dm='y'

np=2048 dmm='w'

sw = sw from ¹³C reference spectrum dmf=9900

ss=2 dpwr=30

tpwr = listed at console sw1 = sw from ¹H reference spectrm

pw = ¹³C 90° pulse width ni = multiple of 64

d1 = ¹H relaxation delay (1-2 * ¹H T₁) dp='n'

d2=0 hs='yn'

presat='n' hst=0.01

phase=1,2 jcc=40

tof = tof from ¹³C reference spectrum rf1 = rf from ¹H reference spectrum

nt = multiple of 4, maximum of 64 rfp1 = rfp from ¹H reference spectrum

ct = set by computer wp1 = wp from ¹H reference spectrum

sp1 = sp from ¹H reference spectrum

Change ni, nt, and d1 to suit your scheduled time. The vertical (non-acquisition) resolution equals sw1/2ni. Typical values are listed below for sw1=25000:

d1	ni	nt	time	vert. res	d1	ni	nt	time	vert. res
1.5	128	32	3:32	195 Hz	1.5	128	128	14:10	195 Hz
1.5	256	32	7:06	98	1.5	256	128	28:22	98
1.5	512	32	14:14	49	1.5	512	128	56:55	49

Processing, Displaying, and Plotting:

Process using the procedure for Processing a 2D Phase-Sensitive Experiment. Plot Pos. only contours.