IU NMR Facility Users Guide

Procedures for
Spectrometer Operation , Data Acquisition , Data Processing , Printing Instructions , & Miscellaneous Notes

Spectrometer Operation

1. Calibrating a 90° pulse

2. lashing the Inova spectrometer for observing and decoupling different combinations of nuclei

3. locking and shimming

4. shimming

5. Estimating Relaxation Times

6. How to Reboot the Spectrometers

7. Variable Temperature Operation

8. Variable Temperature Operation #2

9. I500 Bio Setup

10. "mref" – a new macro for transferring parameters.

Data Acquisition

1D NMR Experiments

1. The basic parameters for a 1D experiment

2. How to add scans to a current experiment

3. 1H one-pulse experiment

4. 13C one-pulse experiment

5. Spin decoupling

6. Signal Suppression

7. 1D NOE experiment

8. DEPT

9. C13DEPT

10. Jvert

11. T1 inversion-recovery experiment

12. 1H with 19F decoupling

13. 19F with 1H decoupling

14. homonuclear decoupling on X nuclei

15. 13C with 1H and 2H decoupling

16. various other double resonance experiments

2D NMR Experiments

    General 2D setup Procedure:
        1) Acquire a 1H spectrum with a 90 degree pulse width (not the
        default of 45 degrees), phase and reference it.
        2) Optimize the sweep width (sw) of your spectrum using "movesw"
        and acquire again.
        3) Copy the parameters to another experiment with mp(1,2), for
        example, if you want to preserve the 1D spectrum.
        4) Join the experiment to which you just moved the parameters.
        5) Choose the 2D experiment you want to run from the Setup menu or
        type the name in directly (ie. "iugcosy") if you know it.

    To view and/or print out more explicit, manual setup procedures for 2D Experiments click here.

Data Processing

1. Optimizing 1D spectra

2. Processing 2D spectra

3. Linear Prediction

4. Baseline Correction

5. Spin Simulation

6. Peak Picking Tips

7. Integration Normalization

8. Integration Tips

9. nOe Calculator

Printing Instructions

1. How do I specify the font and line thickness of my plot?

2. How to make a whitewashed plot?

3. How do I print a spectrum with different colored lines?

4. How do I print a stacked spectrum with multi-colored lines?

5. How do I print Several Different Spectra onto one page?

6. How do I place an inset into my spectrum and print it out?

7. How do I create a Postscript file?

8. How do I place a NMR spectra into a Word document using a Macintosh?

9. How do I import a NMR spectra into a Word or Power Point document using a PC?

10. How do I place a NMR spectra into a Word document using a PC?

11. How do I plot a 2D homonuclear correlation (COSY, NOESY, TOCSY, etc.) type spectrum?

12. How do I plot a 2D heteronuclear correlation (HETCOR, HMQC, HMBC, etc.) type spectrum?

13. How do I print nice line lists on my spectra?

Miscellaneous Notes

1. Guide to MestRe-C a processing and plotting program

2. Guide to SpinWorks a spectral simulation program

3. Guide to gNMR spectral simulation program

4. Guide to C13 Module for calculting 13C chemical shifts

5. How Accurate is the NOE?

6. Product Operators for COSY experiment

7. Relationship between Pulse Widths, Power, and Field Strength

8. How to Save Parameters

9. How to Change your Password

10. 19F chemical shifts and coupling constants

11. 31P chemical shifts and coupling constants

12. Solvents

13. Using the Wavetek and Oscilloscope to Tune and Match a Probe

14. Auto-Shimming Using A Pulsed Field Gradient Along The Z-Axis

15. VNMR on an SGI System. How Do I Do It?

16. Converting VNMR spectra to DNMR5 format

17. How to back up your NMR data

18. How Do I Fix A Corrupt Global File or Experimental Area?

Other NMR sites

1. Queensland University NMR tutorial

2. Queensland University NMR tutorial 2d analysis

Back to NMR Facility Home Page
Send comments to chemnmr@indiana.edu

Last updated: August 21th, 1998
URL: http://nmr.chem.indiana.edu/NMRguide/index.html
Copyright 1996, The Trustees of Indiana University