IU NMR Facility

The NMR facility will be hosting a Bio-NMR Workshop on two successive Fridays next month— November 7 and 14 (see the flyer).

The workshop runs from 9:00 am to 12 noon, and is broken down into a presentation block (9 am-11 am, SI 030) and a hands-on demonstration at the 800 MHz spectrometer (11 am-12 noon, SI 037). Enrollment is limited to 12 people for each of the two sessions, first come, first served!

Students will learn about the nuts and bolts of how biological NMR works, to sample preparation hints, and will emphasize the types of experiments that can be done using this method. The sensitivity of modern NMR spectrometers has improved dramatically so that relatively dilute solutions of purified protein or protein assemblies can be studied. Furthermore, it is now possible to study large systems (up to 100 kD) using this method.

Please send an email to Hongwei if you would like to register for the workshop. Include your name and PI. There is no registration fee and extensive prior knowledge of NMR spectroscopy will not be assumed.

We recently upgraded the FTS cooling system of the I500 to a high-capacity model (the same model as the I400 has). Users now can do low temperature experiments on the 500 down to -55 °C without the need of liquid nitrogen.

Please always make sure the currently installed probe as well as the solvent of your samples can handle the temperature you’re going to set. And, remember to use set_temp command to accurately set the temperature (see the previous post). Talk to the NMR staff if you have questions or need help.

Gem300 has been retired.

A problem on the I500 variable temperature controller has been fixed.

We recently upgraded our Mestrenova program to its latest version 9.0.1. To install the new version, uninstall old versions first, then go to ITG’s software page and click Install It. You will be given instructions and can download the software from there. You don’t have to download and install the license files, since your old license files work for the new version.

Check out the new features of 9.0.1. We have access to the qNMR and Reaction Monitoring plugins.

The pulse gradient driver on I500 has been replaced. It is now possible to do gradient versions of 1D and 2D experiments. However, if you wish to do diffusion experiments, contact NMR staff for assistance.

The pulse gradient driver on the I500 is currently not working.  Gradient versions of 1D & 2D experiments and diffusion experiment are not available at this moment.

Mestrelab will offer a free webinar titled “Reaction Monitoring by NMR: getting the most from the data” on April 23rd, Wednesday, at 10am EDT. If you’re interested, follow this link to register.

Frank will be attending 55th Experimental NMR Conference and Agilent User Meeting from March 21st to 28th.

Actual sample temperature could be different from displayed temperature (or setting temperature if the temperature has been regulated), especially at very high or very low temperature. We recently re-calibrated the sample temperature for all spectrometers, and wrote a macro command set_temp to let users read the actual temperature of samples and set the desired sample temperature accurately.

• To read the actual sample temperature of the current setting, type set_temp.
• To set the sample temperature to 40 °C (actual), type set_temp(40). This sets the parameter temp to a value calculated based on the temperature calibration. You will still need to click Regulate Temp on Spin/Temp page (or type su) to initiate the temperature change.

A short manual for set_temp can be displayed on Text Output page by typing man(‘set_temp’).