BioNMR Documentation

NMR Sample Preparation

  1. Optimization of protein NMR sample buffer conditions
  2. 1L M9 minimal medium recipe
  3. Example protocol for 1L M9 minimal medium cell culture for the N15 or N15/C13 labeled protein

Acquisition on the Bruker AVANCE NEO 600

  1. How to set up expt. on the Bruker Avance Neo 600
  2. How to optimize the transmitter offset (o1) using gs mode or popt
  3. How to process 3D data with NUS on the Bruker 600 with topspin4
  4. How to process NUS 2D spectra collected from B600
  5. How to run NHnoe on B600
  6. How to run N15 T1 on B600
  7. How to run N15 T2 on B600
  8. sofast package setup notes on B600
  9. Using stdisp to calculate the power level for a shape pulse

NMR Data Processing and Analysis

  1. Transferring data to NMRbox
  2. How to extract R1, R1rho, and R2 rates from the relaxation data
  3. Procedures for protein NMR structure calculation by Cyana

NMR pulse sequence blocks

  1. Basic elements: spin echo (Hahn echo), INEPT, refocused INEPT, reverse INEPT, HSQC, PEP-HSQC, S3CT etc.
  2. Gradient pulses: coherence selection, water (solvent) suppression, homospoil (purging unwanted magnetization).

Old Varian notes

  1. Download NMR operation training protocol and the most common commands list here (pdf).
  2. Agilent v800 coldprobe temperature calibration data sheet is here(xls).
  3. Solvent suppression in 1D proton NMR
  4. STD NMR
  5. Modified STD3 pulse sequence Setup and Data Process
  6. NUS 3D experiments set up notes
  7. NUS 3D NMR spectra process notes
  8. BEST-HNCA exp. setup note
  9. The 600 and 800 NMR spectrometers setting notes
  10. Methyl sidechain S2 measurement
  11. Process the spectra from gNhsqc_IPAP.c pulse sequence
  12. Collecting and processing other 7 RDCs
  13. Notes for CPMG relaxation dispersion measurements
  14. Note for roughly estimating the protein aggregation state from its diffusion coefficient measured by NMR method
  15. How to analyze and simultaneously do four curves nonlinear fitting for  methyl C13-ZZ exchange data

BioNMR schedule

  1. google calendar user ID: iubionmr
  2. IU NMR:  V800 and  V600

Updated on 7/3/2025

Comments are closed.